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KMID : 0880220080460050542
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Journal of Microbiology
2008 Volume.46 No. 5 p.542 ~ p.548
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Genome-Wide Transcriptional Responses to Sulfite in Saccharomyces cerevisiae
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Park Hoon
Hwan Yoon-Sun
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Abstract
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Sulfite is a commonly used preservative in foods, beverages, and pharmaceuticals because it is toxic to many microorganisms. In order to understand the global response of Saccharomyces cerevisiae to sulfite, genome-wide transcript profiling following sulfite exposure was obtained. The transcription levels of 21 genes were increased more than 2-fold, while those of 37 genes decreased to a similar extent. Genes involved in carbohydrate metabolism represented the highest proportion of induced genes, which may account for the easily acquired resistance to sulfite. Most of down-regulated genes are involved in transcription, protein biosynthesis, and cell growth. The down-regulation of these genes is thought to reflect growth arrest which occurs during sulfite treatment, allowing cells to save energy. Cells treated with sulfite generated more than 70% of acetaldehyde than untreated cells, suggesting that the increased acetaldehyde production is correlated with the induction of PDC1 gene encoding pyruvate decarboxylase.
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KEYWORD
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DNA microarray, gene expression, sulfite, yeast
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